Atcc hl-60

DNA taken from HL-60 (ATCC® CCL-240 ™) Cell Line. Cell Line Description: The HL-60 cell line is a human acute myeloid leukemia cell line. The HL-60 cell line is included in the genetic alteration cell panel MYC (ATCC® TCP-1035 ™). Spin down before use. Verified mutation in parental cell line: MYC amplification. Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service …

DNA. Protein. ref. consensus. comment HL-60, CCL-240, R248L, 1018.

11.04.2021

Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 2 PubMed citations. Cell culture Human acute promyelocyte leukemia HL-60 cells and acute myeloid leukemia KG1a cells were purchased from the American Type Culture Collection (ATCC)(Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 U/mL, Gibco), and streptomycin (100 µg/mL, Gibco). .. Article Title: A Glycovariant of Human CD44 is … 25/05/2017 ATCC is the complete solution supplier for toxicology From basic research through discovery and development to product testing −Continuous cell lines −Primary cells −hTERT-immortalized primary cells −upcyte ® hepatocytes (ATCC ® ACS-9000 ™) Portfolio features −Reliability −Fully characterized cells −Optimized growth protocols −Scalabilty into all aspects of the toxicology workflow −Biological … The prostate cancer cell lines LNCaP (ATCC, Manassas, VA, USA; ATCC CRL-1740) and PC3 (ATCC CRL-1435) as well as the myeloid cell lines THP-1 (ATCC TIB-202) and HL-60 (ATCC CCL-240) wereusedinthisstudy. HL-60 is a promyelocytic cell line derived by S.J. Collins, et al.

The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI

I cryopreserved HL-60 cells in FBS/ 10% DMSO but unfortunately cannot recover these cells properly. The cells have a good appearance at day 1 and 2.

HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

To ATCC Valued Customers, HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at a concentration of 190 nM. hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT.

Eos-HL-60 . 96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR .

Characteristics. Derivation. HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood Clone 15 HL-60 (ATCC® CRL-1964™). This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with DNA purified from ATCC CCL-240, HL-60, acute promyelocytic leukemia cell line .

HL-60 cells were commonly cultured in RPMI 1640 medium supple-mented with 10% (v/v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 When unopsonized, Wolinella recta ATCC 33228 significantly suppressed the net proliferation of uninduced HL-60 cells, Actinobacillus actinomycetemcomitans strain Y4 was markedly lethal to the cells, and Bacteroides gingivalis ATCC 33277 had no effect. Oct 01, 2012 · HL-60 cells were obtained from the American Type Culture Collection (ATCC) and cultured in IMDM medium with 10% fetal bovine serum (FBS) at 37°C in a fully humidified atmosphere with 5%CO 2. Half of fresh medium were replaced every other day. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in 1640 RPMI Apr 15, 2010 · HL-60 cells (CCL-240) and U-937 cells (CRL-1593.2) were supplied by American Type Culture Collection (ATCC).

3. profiling Clone 15 HL-60 98052918 Human … Clone 15 HL-60 Other Collection No.: ATCC CRL-1964 Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: Clone 15 HL-60 (ECACC 98052918) Keywords: Human caucasian promyelocytic leukaemia Cell Line Description: The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated … 22/05/2018 Compound Viability screens Drug sensitivity AUC (Sanger GDSC1) Drug sensitivity AUC (Sanger GDSC2) Drug sensitivity IC50 (Sanger GDSC1) Drug sensitivity IC50 (Sanger GDSC2) Drug sensitivity AUC (CTD^2) Drug sensitivity replicate-level dose (CTD^2) Drug sensitivity replicate-level dose (Sanger GDSC1) Drug sensitivity replicate-level dose (Sanger GDSC2) This reference provides a recommended procedure to transfect plasmid DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine® LTX Reagent (Cat. No. 15338-100). Important Guidelines for Transfection Follow these important guidelines when transfecting DNA into HL-60 cells using Lipofectamine® LTX Reagent: The addition of antibiotics to media during transfection … Always get your cells from the source (ATCC for the HL-60xs or DSMZ for the PLB-985s).

Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. To ATCC Valued Customers, HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Subsequent exposure of the HL-60/MX1 cells to higher concentrations of mitoxantrone led to the emergence of cells capable of growth at a concentration of 190 nM. hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months.

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May 8, 2015 HL-60(TB), Leukemia, 28.6, 40000. K-562, Leukemia, 19.6, 5000 A549/ATCC, Non-Small Cell Lung, 22.9, 7500. EKVX, Non-Small Cell Lung

96100920 .

HL-60 has recently been reported to contain a sequence similar to that of the human endogenous retrovirus H/F-expressing cell line Reh (ATCC CRL-8286) (70), although there is currently no evidence that human endogenous retroviruses are expressed.

Clone, HL-60, HL60 Human caucasian promyelocytic leukaemia The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid.

In this study, we present the underlying genome and epigenome architecture of HL-60/S4 through its differentiation.